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Old 04-01-2009
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Jessica W is on a distinguished road
the end products of tryptophan degradation are indole and pyruvic acid.
Why do we test for the presence of indole rather than pyruvic acid as the indicator of tryptophanase activity?
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Old 04-01-2009
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Indole is a specific product of tryptophan degradation whereas pyruvic acid is a relatively common biochemical involved in several enzymic pathways including the end product of glycolyis. So, if you measured pyruvic acid the background (blank) level would be very high so measuring indole production is more accurate especially since the determination is linked to a colored product for easy identification.
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